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Using Antibodies: A Lab Manual

ISBN-10:0-87969-544-7
ISBN-13:978-0-87969-544-6

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Description

General descriptionHarlow and Lane have revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been rewritten, reorganized, and updated to provide background, context and step-by-step instructions. Techniques range from choosing the right antibody and handling it correctly to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, this title instead identifies in each case the best approach to specific problems.

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publication infoE. Harlow and D. Lane, Cold Spring Harbor Laboratory Press, 1999, 495 pp., comb bound

Table Of Contents

Table of ContentsChapter 1: Antibody Structure And Function,
Chapter 2: Antibody–Antigen Interactions,
Chapter 3: Choosing Antibodies,
Chapter 4: Handling Antibodies, Storing Antibodies, Purifying Antibodies, Labeling Antibodies,
Chapter 5: Staining Cells, Major Constraints, Choosing The Correct Antibody, Protocols For Immunostaining Of Tissue Culture Cells, Variations On The Immunostaining Procedure,
Chapter 6: Staining Tissues, Major Constraints, Choosing the Correct Antibody, Immunostaining Tissues With Polyclonal Or Monoclonal Antibodies, Protocols For Staining Tissue Sections, Variations For Staining Yeast, Variations For Staining Drosophila, Protocols For Antigen Retrieval,
Chapter 7: Immunoprecipitation, Major Constraints, Choosing The Correct Antibody, Immunoprecipitation Protocols, Variations On The Immunoprecipitation Procedure,
Chapter 8: Immunoblotting, Major Constraints, Choosing The Correct Antibody, Immunoblotting Protocols, Variations In The Detection Methods, Variations In The Immunoblotting Technique,
Chapter 9: Immunoaffinity Purification, Major Constraints, Choosing The Correct Antibody, Immunoaffinity Purification With Polyclonal And Monoclonal Antibodies, Protocols For Immunoaffinity Purification, Variations On Immunoaffinity Purification,
Chapter 10: Tagging Proteins, Major Constraints, Why Tag Your Protein, Tags For Protein Detection, Tags For Protein Purification, Choosing The Right Tag, Deciding Where To Position The Tag, How To Tag Your Protein,
Chapter 11: Epitope Mapping, Determining The Structural Requirements Of An Epitope, Choosing An Epitope-mapping Method, Mapping By Competition Assay, Mapping By Expression Of Gene Fragments, Mapping Using Synthetic Peptides, Synthesis Or Purchase Of The Peptide Set, Alternate Approaches To Epitope Mapping, Appendix I: Electrophoresis,
Appendix II: Protein Techniques,
Appendix III: General Information,
Appendix IV: Cautions,
Appendix V: Trademarks,
Appendix VI: Suppliers,